Characteristics of Na+/K+-ATPase mediated proton current in Na+- and K+-free extracellular solutions. Indications for kinetic similarities between H+/K+-ATPase and Na+/K+-ATPase
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Regulation of NaK-ATPase Activity by Neurotransmitters
The hitherto unknown NT-regulated mechanism of NaK-ATPase localized in the nerve ending membranes is found. The mechanism certainly has a functional significance and must be involved in the regulation – modulation of chemical synaptic transmission. On the other hand , the availability of the discovered specific protein, regulators (SFa and SFi) of synaptic origin, makes it possible to consider ...
متن کاملStructural similarities of Na,K-ATPase and SERCA, the Ca(2+)-ATPase of the sarcoplasmic reticulum.
The crystal structure of SERCA1a (skeletal-muscle sarcoplasmic-reticulum/endoplasmic-reticulum Ca(2+)-ATPase) has recently been determined at 2.6 A (note 1 A = 0.1 nm) resolution [Toyoshima, Nakasako, Nomura and Ogawa (2000) Nature (London) 405, 647-655]. Other P-type ATPases are thought to share key features of the ATP hydrolysis site and a central core of transmembrane helices. Outside of the...
متن کاملLocalization of NaK ATPase on cultured human retinal pigment epithelium.
PURPOSE To localize NaK ATPase sites on cultured human retinal pigment epithelium (RPE). METHODS Cultured human RPE from fetal, 2-year-old, and 21-year-old donors was grown to confluence in microporous culture wells for 4 months to 2 years, mounted in a small-volume Ussing chamber, and perfused with growth medium. Ouabain (10(-5)-M) was applied to the basal and apical sides of the RPE. Change...
متن کاملLead ion activates phosphorylation of electroplax Na+- and K+-dependent adenosine triphosphatase ((NaK)-ATPase) in the absence of sodium ion.
PbCl, in micromolar concentrations stimulates phosphorylation of electroplax microsomal protein in the absence of Nat. Other divalent cations showed little or no such effect. The (Mg’+ + Pb’+)and (Mg”+ + Na+)-dependent membrane-bound protein kinase activities in electroplax particulate preparations exhibit properties in common, including their acid stability, ouabain sensitivity, ATP specificit...
متن کاملEssential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3
1 A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3* Background: Neurons express two Na,K-ATPase isoforms, the ubiquitous α1 and neuron-specific α3. Result: α3 is important for control of membrane potential and is fully responsible for restoration of large [Na + ] i increases. Conclusion: α1 and α3 are required for basal neuronal function, but α3 controls resto...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1996
ISSN: 0005-2736
DOI: 10.1016/0005-2736(96)00057-0